Charles River: Research Models and Services: Transgenic Services: Cryopreservation Quote

Research Models and Services

Cryopreservation Quote

Contact information for cryopreservation quote:

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US
Non-US

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Please describe the animals you will send for cryopreservation.

Species	Strain/Line	Zygosity	Sex	Age	Qty

* Total number of cages to be sent:

Background Strain(s):

Please describe any phenotypes to be expected that may affect the health or breeding ability of the animals:

In order to initiate mouse embryo cryopreservation, we require 5-8 proven breeder males, less than 6 months of age, and 3-4 week old females in batches of 20 females supplied to us biweekly for project duration. In certain cases, as few as 2 proven breeder males may be acceptable. For rat embryo collections we require 8-10 breeder males, less than 6 months of age, and 10-12 week old females in batches of 20 females supplied to us biweekly for project duration. Depending on the quantity of animals available, mating scheme, and reproductive performance of the strain, we will determine whether to collect embryos via live matings or in vitro fertilization (IVF).

I do not have the desired number of transgenic/knockout animals available, please initiate expansion breeding program at Charles River Laboratories to supply project animals

I have the desired number of animals available and will supply:

Transgenic/knockout males as well as transgenic/knockout females
Transgenic/knockout males only and I will use wild type/inbred/outbred/hybrid females instead;

I would like to cryopreserve sperm from transgenic/knockout males

Mating scheme for embryo collections:

Homozygous male x homozygous female (200 embryos)

Homozygous male x wild type female (200-275 embryos)

Heterozygous male x wild type female (200-275 embryos)

Other

Strain of wild type females:	Source:
C57BL/6 Other:	Charles River Laboratories Other:

Would you like to freeze a different number of embryos that the Charles River recommendation? Yes
If yes, quantity to cryopreserve:

Embryo storage:

at Charles River Laboratories
at customer's facility
split between Charles River Laboratories / customer

Upon project completion:

all remaining animals returned to client
all remaining animals euthanized

Additional information: