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Complete Monoclonal Antibody Production Packages
BASIC PACKAGE
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Immunization*
Immunization of 3 BALB/c mice with 4 injections within 3 months;
initial screening and evaluation of serum titer by ELISA
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Fusion
Spleen cell preparation from animal with the best response against the
antigen, fusion, distribution of hybridoma cells into 96-well plates, screening
of hybridomas - 10 plates total
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Analysis
Detection of specific antibody in culture medium, ELISA with 1 antigen,
delivery of 20 culture supernatants of hybridoma cells with the best response
against the antigen; cultivation of 5 positive clones, delivery of 5 vital or
cryopreserved clones
*If no animal produces sufficiently high titers due to low immunogenicity
of the antigen, the production process can be ended here without further
costs.
STANDARD PACKAGE
-
Immunization*
Immunization of 3 BALB/c mice with 4 injections within 3 months; initial
screening and evaluation of serum titer by ELISA.
-
Fusion
Spleen cell preparation from animal with the best response against the
antigen, fusion, distribution of hybridoma cells into 96-well plates, screening
of hybridomas - 20 plates total
-
Analysis
Detection of specific antibody in culture medium, ELISA with 1 antigen,
delivery of 30 culture supernatants of hybridoma cells with the best response
against the antigen; cultivation of 10 positive clones, delivery of 10 vital or
cryopreserved clones
*If no animal produces sufficiently high titers due to low immunogenicity
of the antigen, the production process can be ended here without further
costs.
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